Home > Teams > Structural microbiology and enzymology (S. Moréra)
Structural microbiology and enzymology

 

Group leader: Solange Moréra This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

Theme

Our team is interested in elucidating action mechanisms of singles enzymes or within multi-molecular complexes. Our work allows supplying fundamental basis describing the enzyme’s molecular function. It will lead to the elaboration of new antimicrobial or new anticancerous agents (drug design) for our enzymes of interest which are considered as potential therapeutic targets. Our team possesses a strong expertise in structural analysis by crystallography of enzymatic mechanisms as the transfer of phosphate and sugar. We are also specialized in the study of protein-protein interactions, protein-DNA and protein-ligand interactions which are at the center of the biological processes we are interested in. Our main themes concern:

  • Bacterial or viral proteins mainly involved in host-pathogen relationship.
  • The molecular chaperon Hsp90 and its machinery through the study of Hsp90 and the R2TP complex formed by two helicases and two co-chaperons of Hsp90.
  • DNA modification or DNA repair enzymes using the base flipping mechanism

 

Experimental approach

We use protein crystallography to determine the 3D structure of proteins and analyze their function alone or within multimolecular assemblies. The structure-function analysis is completed by biochemical and biophysical studies in solution. Techniques usually used are:

  • Production of proteins (cloning, mutagenesis, bacterial culture…)
  • Purification of recombinant proteins (Chromatography of affinity, Gel filtration…)
  • Enzymatic tests and inhibition measurement ( Spectrophotometry)
  • Crystallization, X-rays crystallography and structural analysis
  • Interactions determination (Microcalorimétrie, Fluorescence, BIAcore)
  • Conformational analyses (Circular Dichroism, Ultracentrifugation, light scattering)
  • Post-traductionnal modifications analysis (mass spectrometry)
  • Bioinformatic and molecular modelisation

 

Current Collaborations

  • Periplasmic  binding protein :
    Denis Faure (ISV, Gif-sur-Yvette), Yves Queneau (ENS, Lyon), John Reader (USA)
  • Hsp90 and co-factors :
    Edouard Bertrand  (IGM, Montpellier) ;  Christiane Branlant/Bruno Charpentier (MAEM, Nancy) ; Sarah Sanglier (LSMBO, Strasbourg) ; Philippe Meyer (IBPC, Paris)
  • DNA enzymes :
    Murat Saparbaev (IGR, Villejuif) ; Saulius Klimasauskas (Vilnius, Lituanie)
 

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1st International Meeting - Building The Cell - 24th-26th September 2014
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Année internationale de la cristallographie: des équipes du LEBS à la une  
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Roman Gorelik, lauréat du prix Hélène Stark de la Fondation ARC
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Irène Dang, lauréate de la bourse L'Oréal ...
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